CRYOSURVIVAL OF GOAT SPERMATOZOA IN THREE DIFFERENT TRIS BASED EXTENDERS SUPPLEMENTED WITH VITAMIN E

• E. O. Adekunle

  Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

  Author

• M. O. Logunleko

  Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

  Author

• B. C. Majekodunmi

  Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

  Author

• J. O. Daramola

  Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

  Author

• S. O. Sowande

  Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

  Author

• M. O. Onagbesan

  Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

  Author

Tris egg yolk(TEY), Tris coconut water (TCW) and Tris skim milk (TSM) extenders supplemented with 2, 4, 6 and 8 mM of vitamin E were studied for their cryosurvival potential on spermatozoa of West African Dwarf (WAD) goat bucks. Semen samples were diluted with the vitamin E supplemented extenders and cryopreserved for 30 days in liquid nitrogen. Spermatozoa with 6 mM and 8 mM vitamin E in TCW and 8 mM vitamin E in TEY and TSM extenders had higher (P<0.05) motility. . Spermatozoa cryopreserved with 6 mM and 8 mM vitamin E in TCW had higher (P<0.05) membrane integrity with lower (P<0.05) abnormality compared to other extenders. Malondialdehyde (MDA) concentration was reduced in TCW and TEY extenders supplemented with 6 mM and 8 mM vitamin E. TCW and TSM extenders supplemented with 6 mM and 8 mM vitamin E had higher (P<0.05) spermatozoa that underwent acrosome reaction and capacitation. Inclusion of vitamin E inTCW and TSM based extenders promoted sperm viability,acrosome reaction and sperm capacitation of WAD goat buck spermatozoa indicating that spermatozoa obtained from WAD bucks were better preserved in vitamin E supplemented TCW and TSM based extender.

1. E. O. Adekunle, Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

   Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

2. M. O. Logunleko, Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

   Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

3. B. C. Majekodunmi, Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

   Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

4. J. O. Daramola, Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

   Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

5. S. O. Sowande, Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

   Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

6. M. O. Onagbesan, Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

   Department of Animal Physiology, Federal University of Agriculture, Abeokuta, Nigeria

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