Performance of Diets for Oreochromis niloticus and Tilapia zillii by Inclusion of Cassava Leaf Protein Concentrate as Partial Replacement for SolventExtracted Soybean Meal

• O. Fagbenro

  Department of Fisheries and Aquaculture Technology, The Federal University of Technology, PMB 704 Akure, Ondo State, Nigeria

  Author

• A. Oresegun

  Department of Biotechnology /Fish Nutrition, Nigeria Institute for Oceanography and Marine Research, PMB 12729 Victoria Island, Lagos State, Nigeria.

  Author

• L. Nwanna

  Department of Fisheries and Aquaculture Technology, The Federal University of Technology, PMB 704 Akure, Ondo State, Nigeria

  Author

• P. Ilona

  International Institute for Tropical Agriculture, Ibadan, Oyo State, Nigeria.

  Author

• O. Olurotimi

  Department of Fisheries and Aquaculture Technology, The Federal University of Technology, PMB 704 Akure, Ondo State, Nigeria

  Author

• A. Adebayo

  Department of Fisheries and Aquaculture Technology, The Federal University of Technology, PMB 704 Akure, Ondo State, Nigeria

  Author

Cassava (cultivar TME419) leaves were processed into leaf protein concentrate and evaluated in diets (320 g/kg protein, 80 g/kg lipid, 19.5 MJ/kg gross energy) fed to Oreochromis niloticus (3.2±0.3 g) and Tilapia zillii (3.3±0.4 g). A control diet (TD1) contained solvent-extracted soybean meal (SBM), which was substituted at 20%, 40%, 60% or 80% with the cassava leaf protein concentrate (CLPC) in test diets TD2, TD3, TD4 or TD5, respectively. Fishes were assigned in triplicate diet treatments (60 fish/treatment) in a complete randomized design (60 fish/treatment) and fed to apparent satiation twice daily for 70 days. Data were analysed statistically using one-way analysis of variance test. No fish mortality occurred in all treatments. Values obtained for growth response indices (mean weight gain, percentage weight gain) were statistically similar (p >0.05) as CLPC substituted up to 60% and 80% of SBM in diets for O. niloticus and T. zillii, respectively, and there were good growth response and diet utilization. Fish growth declined and diet was poorly utilized beyond 60% or 80% these inclusion levels, caused by reduction in protein and energy digestibility. O. niloticus fed with diet TD5 showed slight histological alterations in livers; caused by residual anti-nutrients while T. zillii showed no alterations or abnormalities. Erythrocyte sedimentation rate was significantly higher (p <0.05) in O. niloticus fed with diet TD5, while haematocrit, haemoglobin concentration, erythrocyte and leucocyte counts were statistically similar (p >0.05). The suitability of CLPC as dietary protein for O. niloticus will depend on further reduction/removal of inherent anti-nutrients as well as improving digestibility of nutrients.

1. O. Fagbenro, Department of Fisheries and Aquaculture Technology, The Federal University of Technology, PMB 704 Akure, Ondo State, Nigeria

   Department of Fisheries and Aquaculture Technology, The Federal University of Technology, PMB 704 Akure, Ondo State, Nigeria

2. A. Oresegun, Department of Biotechnology /Fish Nutrition, Nigeria Institute for Oceanography and Marine Research, PMB 12729 Victoria Island, Lagos State, Nigeria.

   Department of Biotechnology /Fish Nutrition, Nigeria Institute for Oceanography and Marine Research, PMB 12729 Victoria Island, Lagos State, Nigeria.

3. L. Nwanna, Department of Fisheries and Aquaculture Technology, The Federal University of Technology, PMB 704 Akure, Ondo State, Nigeria

   Department of Fisheries and Aquaculture Technology, The Federal University of Technology, PMB 704 Akure, Ondo State, Nigeria

4. P. Ilona, International Institute for Tropical Agriculture, Ibadan, Oyo State, Nigeria.

   International Institute for Tropical Agriculture, Ibadan, Oyo State, Nigeria.

5. O. Olurotimi, Department of Fisheries and Aquaculture Technology, The Federal University of Technology, PMB 704 Akure, Ondo State, Nigeria

   Department of Fisheries and Aquaculture Technology, The Federal University of Technology, PMB 704 Akure, Ondo State, Nigeria

6. A. Adebayo, Department of Fisheries and Aquaculture Technology, The Federal University of Technology, PMB 704 Akure, Ondo State, Nigeria

   Department of Fisheries and Aquaculture Technology, The Federal University of Technology, PMB 704 Akure, Ondo State, Nigeria

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